This is an exceptional Meiji Period Japanese ambrotype portraying two young men and a boy. Based on their appearance, they are probably brothers. The older brother are wearing the traditional Japanese garb with the oldest holding a floppy, western styled hat. What makes this image truly unique is that the very dark skinned boy is wearing a western designed uniform and cap! Unfortunately there is no kanji script associated with the photograph that might explain the image.
The ambrotype comes in a handcrafted polonia or kiri wood, full case (Berg 6-138, VR p. 460) with an unusual makers mark on the top (probably identifying the maker), indicating an original matched case and cover. The image measures 2.5 x 3.75 inches and the case is 3 x 4.3 inches. The photograph has nice clarity and tonality. Both the ambrotype and case are in great condition.